Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Enhanced activation of human dendritic cells by silencing SOCS1 and activating TLRs simultaneously

doi: 10.1007/s00262-012-1218-4

Figure Lengend Snippet: Phenotypic analysis of DCs. At 24 h after nucleofection, DCs were harvested and stained with mAbs against DCs surface markers or appropriate isotype control antibody as described in “Materials and methods”. Percentage of positive DCs is indicated. One representative experiment of five is shown

Article Snippet: Nucleofection DCs were harvested at day 6 of culture, washed once in PBS/1%BSA and resuspended in specified nucleofection buffer from Amaxa (Germany) at a final concentration of 2 × 10 7 cells/ml.

Techniques: Staining, Control

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Enhanced activation of human dendritic cells by silencing SOCS1 and activating TLRs simultaneously

doi: 10.1007/s00262-012-1218-4

Figure Lengend Snippet: Th1 cytokines secreted by pshS1NH–DCs were higher than those secreted by non-nucleofected DCs and pNC-GFP-DCs. 1 non-nucleofected DCs; 2 pNC-GFP-DCs; 3 pshS1NH–DCs. At 48 h after nucleofection, supernatants were collected for TNF-α, IL-6, IL-1β and IL-12p70 ELISA analyses. The pshS1NH–DCs secreted significantly higher level of Th1 cytokines than the pNC-GFP-DCs and non-nucleofected DCs (P < 0.05). Data shown are the means ± SEM and representative of six independent experiments. The significant differences between pshS1NH–DCs and control were determined by ANOVA

Article Snippet: Nucleofection DCs were harvested at day 6 of culture, washed once in PBS/1%BSA and resuspended in specified nucleofection buffer from Amaxa (Germany) at a final concentration of 2 × 10 7 cells/ml.

Techniques: Enzyme-linked Immunosorbent Assay, Control